Journal: Journal of Translational Medicine

Article Title: Astrocyte elevated gene-1 (AEG-1) is a marker for aggressive salivary gland carcinoma

doi: 10.1186/1479-5876-9-205

Figure Lengend Snippet: Upregulation of AEG-1 expression in SGC tissues . (A and B) Expression of AEG-1 protein and mRNA in 2 normal human salivary gland tissues and 8 SGC tissues by Western blot (A) and real-time PCR (B), respectively. * P < 0.05, ** P < 0.01, *** P < 0.001. (C) Expression of AEG-1 protein in each of the primary SGC tissues (T) and adjacent non-cancerous tissues (N) in the same patient determined by Western blot. (D) Real time-PCR analysis of AEG-1 expression in each of the T and N tissues. GAPDH was used as an internal control. Columns, mean from three parallel experiments; bars, SD. (E) Expression of AEG-1 mRNA in each of the primary SGC tissues (lower panel) and adjacent non-cancerous tissues (upper panel) paired from the same patient determined by IHC.

Article Snippet: Western blots were performed according to standard methods as described previously [ ], using a rabbit anti-AEG-1 polyclonal antibody (1:500; Zymed).

Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction

Journal: Journal of Translational Medicine

Article Title: Astrocyte elevated gene-1 (AEG-1) is a marker for aggressive salivary gland carcinoma

doi: 10.1186/1479-5876-9-205

Figure Lengend Snippet: AEG-1 protein overexpression in archived paraffin-embedded SGC tissue sections as examined by IHC . (A) Representative images from IHC analyses of AEG-1 expression in seven histological types of SGC. (B) Representative images from IHC analyses of AEG-1 expression in normal human salivary gland tissues and primary SGC specimens. (C) Statistical analyses of the average MOD of AEG-1 staining between normal salivary gland tissues (2 cases) and SGC specimens of different clinical stages. * P < 0.05.

Article Snippet: Western blots were performed according to standard methods as described previously [ ], using a rabbit anti-AEG-1 polyclonal antibody (1:500; Zymed).

Techniques: Over Expression, Expressing, Staining

Journal: Journal of Translational Medicine

Article Title: Astrocyte elevated gene-1 (AEG-1) is a marker for aggressive salivary gland carcinoma

doi: 10.1186/1479-5876-9-205

Figure Lengend Snippet: Kaplan-Meier curves with univariate analyses (log-rank) for patients with low AEG-1 expression (bold line) versus high AEG-1 expression tumors (dotted line) . The cumulative 5-year survival rate was 78.4% in the low AEG-1 protein expression group (n = 62), whereas it was only 45.0% in the high AEG-1 expression group (n = 79).

Article Snippet: Western blots were performed according to standard methods as described previously [ ], using a rabbit anti-AEG-1 polyclonal antibody (1:500; Zymed).

Techniques: Expressing

Journal: Journal of Translational Medicine

Article Title: Astrocyte elevated gene-1 (AEG-1) is a marker for aggressive salivary gland carcinoma

doi: 10.1186/1479-5876-9-205

Figure Lengend Snippet: Overall survival curves stratified by AEG-1 levels according to clinical stage, T and M classifications . (A, B) The differences of survival curves according to AEG-1 expression were seen in advanced clinical stage (B), but not in early clinical stage (A). (C, D) In the T3-T4 subgroup, patients with low AEG-1 expression showed significantly better overall survival (D). In the T1-T2 subgroup, the patients' overall survival times were not significantly different between the two groups (C). (E, F) Survival time was longer in patients with low AEG-1 expression regardless of distant metastasis (F). No significant difference between low and high AEG-1 expression groups were found in the M0 subgroup (E).

Article Snippet: Western blots were performed according to standard methods as described previously [ ], using a rabbit anti-AEG-1 polyclonal antibody (1:500; Zymed).

Techniques: Expressing

Journal: Journal of Translational Medicine

Article Title: Astrocyte elevated gene-1 (AEG-1) is a marker for aggressive salivary gland carcinoma

doi: 10.1186/1479-5876-9-205

Figure Lengend Snippet: Upregulation of AEG-1 expression in SGC tissues . (A and B) Expression of AEG-1 protein and mRNA in 2 normal human salivary gland tissues and 8 SGC tissues by Western blot (A) and real-time PCR (B), respectively. * P < 0.05, ** P < 0.01, *** P < 0.001. (C) Expression of AEG-1 protein in each of the primary SGC tissues (T) and adjacent non-cancerous tissues (N) in the same patient determined by Western blot. (D) Real time-PCR analysis of AEG-1 expression in each of the T and N tissues. GAPDH was used as an internal control. Columns, mean from three parallel experiments; bars, SD. (E) Expression of AEG-1 mRNA in each of the primary SGC tissues (lower panel) and adjacent non-cancerous tissues (upper panel) paired from the same patient determined by IHC.

Article Snippet: Briefly, tissue sections were incubated with a rabbit anti-AEG-1 antibody (1:200; Zymed) overnight at 4°C.

Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction

Journal: Journal of Translational Medicine

Article Title: Astrocyte elevated gene-1 (AEG-1) is a marker for aggressive salivary gland carcinoma

doi: 10.1186/1479-5876-9-205

Figure Lengend Snippet: AEG-1 protein overexpression in archived paraffin-embedded SGC tissue sections as examined by IHC . (A) Representative images from IHC analyses of AEG-1 expression in seven histological types of SGC. (B) Representative images from IHC analyses of AEG-1 expression in normal human salivary gland tissues and primary SGC specimens. (C) Statistical analyses of the average MOD of AEG-1 staining between normal salivary gland tissues (2 cases) and SGC specimens of different clinical stages. * P < 0.05.

Article Snippet: Briefly, tissue sections were incubated with a rabbit anti-AEG-1 antibody (1:200; Zymed) overnight at 4°C.

Techniques: Over Expression, Expressing, Staining

Journal: Journal of Translational Medicine

Article Title: Astrocyte elevated gene-1 (AEG-1) is a marker for aggressive salivary gland carcinoma

doi: 10.1186/1479-5876-9-205

Figure Lengend Snippet: Kaplan-Meier curves with univariate analyses (log-rank) for patients with low AEG-1 expression (bold line) versus high AEG-1 expression tumors (dotted line) . The cumulative 5-year survival rate was 78.4% in the low AEG-1 protein expression group (n = 62), whereas it was only 45.0% in the high AEG-1 expression group (n = 79).

Article Snippet: Briefly, tissue sections were incubated with a rabbit anti-AEG-1 antibody (1:200; Zymed) overnight at 4°C.

Techniques: Expressing

Journal: Journal of Translational Medicine

Article Title: Astrocyte elevated gene-1 (AEG-1) is a marker for aggressive salivary gland carcinoma

doi: 10.1186/1479-5876-9-205

Figure Lengend Snippet: Overall survival curves stratified by AEG-1 levels according to clinical stage, T and M classifications . (A, B) The differences of survival curves according to AEG-1 expression were seen in advanced clinical stage (B), but not in early clinical stage (A). (C, D) In the T3-T4 subgroup, patients with low AEG-1 expression showed significantly better overall survival (D). In the T1-T2 subgroup, the patients' overall survival times were not significantly different between the two groups (C). (E, F) Survival time was longer in patients with low AEG-1 expression regardless of distant metastasis (F). No significant difference between low and high AEG-1 expression groups were found in the M0 subgroup (E).

Article Snippet: Briefly, tissue sections were incubated with a rabbit anti-AEG-1 antibody (1:200; Zymed) overnight at 4°C.

Techniques: Expressing